MUTYH mediates the toxicity of combined DNA 6-thioguanine and UVA radiation
نویسندگان
چکیده
منابع مشابه
MUTYH mediates the toxicity of combined DNA 6-thioguanine and UVA radiation
The therapeutic thiopurines, including the immunosuppressant azathioprine (Aza) cause the accumulation of the UVA photosensitizer 6-thioguanine (6-TG) in the DNA of the patients' cells. DNA 6-TG and UVA are synergistically cytotoxic and their interaction causes oxidative damage. The MUTYH DNA glycosylase participates in the base excision repair of oxidized DNA bases. Using Mutyh-nullmouse fibro...
متن کاملCrosslinking of DNA repair and replication proteins to DNA in cells treated with 6-thioguanine and UVA
The DNA of patients taking immunosuppressive and anti-inflammatory thiopurines contains 6-thioguanine (6-TG) and their skin is hypersensitive to ultraviolet A (UVA) radiation. DNA 6-TG absorbs UVA and generates reactive oxygen species that damage DNA and proteins. Here, we show that the DNA damage includes covalent DNA-protein crosslinks. An oligonucleotide containing a single 6-TG is photochem...
متن کاملEffect of 6-Thioguanine and Methyl-6-Thioguanine on stability of DNA duplexes
6-Thioguanine (isG) has been used for the past thirty years as an anti-cancer agent in maintenance chemotherapy for childhood acute lymphoblastic leukemia as well as acute myeloid leukemia. Despite its long-standing clinical use, little is understood of the molecular basis of the cytotoxicity associated with 6SG. Research has shown that 6SG is incorporated into DNA during replication after whic...
متن کاملOxidation-mediated DNA cross-linking contributes to the toxicity of 6-thioguanine in human cells.
The thiopurines azathioprine and 6-mercaptopurine have been extensively prescribed as immunosuppressant and anticancer agents for several decades. A third member of the thiopurine family, 6-thioguanine (6-TG), has been used less widely. Although known to be partly dependent on DNA mismatch repair (MMR), the cytotoxicity of 6-TG remains incompletely understood. Here, we describe a novel MMR-inde...
متن کاملGuanine sulphinate is a major stable product of photochemical oxidation of DNA 6-thioguanine by UVA irradiation
The DNA of patients taking the immunosuppressant and anticancer drugs azathioprine or 6-mercaptopurine contains 6-thioguanine (6-TG). The skin of these patients is selectively sensitive to ultraviolet A radiation (UVA) and they suffer an extremely high incidence of sunlight-induced skin cancer with long-term treatment. DNA 6-TG interacts with UVA to generate reactive oxygen species, which oxidi...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Oncotarget
سال: 2014
ISSN: 1949-2553
DOI: 10.18632/oncotarget.3037